HEATING EFFECT ON PHYTOCHEMICAL AND PROXIMATE CONTENTS OF COOKED AQUEOUS EXTRACT OF PHASEOLUS VULGARIS

(KIDNEY BEANS)

Idoko A*, Onyinye AN, Blessing NO, Ayomide TA, Philip OC, Nwali ON

Department of Biochemistry, Faculty of Natural Sciences, Caritas University, Amorji – Nike, P.M.B. 01784, Enugu, Nigeria.

ABSTRACT

Plant chemicals and nutrients abound in different parts of plants and in different compositions. P. vulgaris (kidney beans) is a nourishing leguminous food commonly eaten by both human beings and animals in the world due to its health benefits and risk reduction of diseases. It is however, indispensible for plants’ food stuff to retain their phytonutrients for maximum benefits. This study therefore investigated the effect of heat on the phytochemicals and proximate contents in cooked P. vulgaris (kidney beans). Qualitative and quantitative phytochemical analysis and proximate (nutrient contents) analysis were determined on fresh kidney bean (FKB) and cooked kidney bean (CKB). Results of phytochemical quantification revealed a significant (p˂0.05) increase of alkaloids and saponins in FKB than those in CKB, significant (p˂0.05) increase of flavonoids, glycosides and tannins in CKB than those in FKB. While proximate analysis of cooked sample (CKB) showed significant (p˂0.05) increase in protein content, crude ash content and carbohydrate content FKB. While moisture content, crude fibre and crude fat of FKB were significantly (p˂0.05) higher than those in CKB of Phaseolus vulgaris. Obviously, the increased concentrations of phytochemicals in fresh Phaseolus vulgaris may be due to the absence of heat action and the heating effect on cooked P. vulgaris could unleash the high rich nutrients value and could supply its antioxidants roles, thereby improving healthy life when eaten cooked.

Keywords: Cooked bean, fresh bean, heating effect, Phaseolus vulgaris, phytochemicals, proximate content.

INTRODUCTION

Kidney bean (P. vulgaris) derived its name from the visual resemblance of the kidney in shape and colour. However, the red colour of the kidney beans should not be confused with other beans such as the pinto beans and adzuki beans, which are also red in colour¹. P. vulgaris beans plant strive best in subtropical and tropical weather. The worlds’ largest producer of beans is Brazil, having approximately five million hectares of land cultivated, with the production of 2.2–2.5 million tons². Researchers have evidently reported the various nutrients in beans and their health benefits, sample recipes and preparation tips³. Beans contain several nutrients including mineral salts such as Mg, Ca, P, Fe and K; contain about 20-25% proteins, 50-60% complex carbohydrates and vitamins⁴. Certain anti nutrients are also found in beans which inhibit and limit absorption of other useful nutrients. They include phytic acid and oligosaccharides; they limit carbohydrate and protein absorption and trypsin, which inhibit tannins absorption⁵. Anti-nutritional contents in beans can modify the bioavailability of iron. Cooking was reported to improve the bioavailability, increases the digestion and absorption of macronutrients and micronutrients found in food due to a soften matrix of the food by heat treatment^6,7. The contents of tannins and phytic acid in beans are reported to bind with some essential dietary minerals and proteins, thus affecting their absorption and bioavailability^8,9. Generally, food cooking has been resourcefully shown to destroy injurious microorganisms and bacteria that may result in food-borne disease due to improper handling¹⁰. The digestion of protein and starch was found to be increased from 25-60% in raw beans to 85% in cooked beans⁷. The sensory properties in beans, including soft texture, mushy texture, sweet, taste and flavor of cooked beans were found to be improved by cooking⁴. Cooking can result in reduction of content of certain vitamins and volatile phytochemicals and also affect the composition of some nutrients such as amino acids and minerals⁴. Soaking (quick, short or long traditional) and cooking of beans can easily result in loss or reduced folate content during interaction with fibers¹¹. However, in order to take full advantage of the natural folate content in beans, it is recommended that the method of routine slow-soak before cooking to prepare the beans within two hours thirty minutes should be adopted¹¹. The folate vitamin B notably in beans or its synthetic equivalent, folic acid serves an important function in the human body especially in red blood cells production and it is also involved in embryonic nervous system development at early stages of pregnancy¹². Cooking was reported to increase the solubility and bioactivity of soluble iron in beans, lentils, legumes and chickpeas¹³. The chemical composition of P. vulgaris (kidney beans) was reported to be affected by cooking. Cooking resulted in destruction of antinutrients, alteration of distribution or bioavailability of iron (in glutelins, globulins, albumins and prolamins) and protein denaturation³. Beans are generally rich in health-rich nutrients and consumption of more beans diets could better in general, the healthiness of a person and eliminate risk of obesity, cancers and heart diseases³. Thus, this study investigated the effect of heat on the phytochemicals and proximate contents in cooked P. vulgaris (kidney beans).

MATERIALS AND METHODS

Collection and Authentication of Bean Seeds

1. vulgaris (kidney beans) seeds were purchased from a commercial market (Ogbete main market) in Enugu state, Nigeria. The seeds were identified and authenticated and a voucher number of UNH no 452 (UNH stands for University of Nigeria Herbarium), was given by Mr. Onyeukwu Chijioke John a plant Taxonomist, Department of Plant Science and Biotechnology, University of Nigeria, Nsukka, Enugu state.

Preparation of Aqueous Extract of Fresh Kidney Bean (FKB) and Cooked Kidney Bean (CKB)

1. vulgaris (kidney bean) was prepared by winnowing, hand picking of stones and removal of dirt and then lightly washed to remove dust and air dried.

Preparation of Fresh Sample (FKB)

Five hundred gram (500g) of the dried bean seeds was weighed and grinded/homogenized into powder. After which it was stored in a clean grease free airtight container with proper labeling for proximate and phytochemicals analysis.

Preparation of Cooked Sample (CKB)

The cooked P. vulgaris (kidney beans) sample was prepared appropriately by hand picking to remove all foreign particles followed by washing and cooked with enough water until soft and without broth to prevent the loss of some phytochemicals in the bean broth. This was dried under mild sunlight for two weeks under strict supervision. Five hundred gram (500g) of the dried bean seeds was weighed grinded/homogenized into powder. After which it was stored in a clean grease free airtight container with proper labeling for proximate and phytochemical analysis.

Preparation of dry extract from samples

From the powdered samples (CKB and FKB), 200g was weighed and soaked in 700ml of distilled water, carefully sealed, left standing for two days (for thorough extraction), before filtering with whatman filter paper. Concentration of the filtrate was achieved in a water bath at temperature of 70ºC.

Qualitative phytochemical screening of Fresh Bean (FKB) and Cooked Kidney Bean (CKB) samples

Alkaloids, flavonoids, saponins, glycosides, phenols, steroids, tannins, reducing sugars and anthraquinones were identified in cooked and fresh kidney beans samples using standard methods described by Harborne¹⁴; Trease and Evans¹⁸ with some modifications.

Quantitative phytochemical screening of fresh Kidney Bean (FKB) and Cooked Kidney Bean (CKB) samples

Alkaloids determination

Alkaloids were determined in the bean samples by the method described by Harborne¹⁴. Five gram (5g) of powered bean sample was put in a 250ml beaker, unto which was added 200ml of 10% acetic acid in ethanol, covered and left to stand for 4 hours at room temperature and then filtered. Concentration of the filtrate was done in a water bath by evaporation and treatment with additional drops of concentrated aqueous ammonium solution until the alkaloid was precipitated. The precipitated alkaloid was put in a filter paper and weighed as W1. Then, 1% NH₃ solution was used to wash the precipitate and even dried at 80ºC. Then the residue and the filter paper after drying and cooling were weighed as W2. Alkaloid in the sample was expressed in % weight of the sample after calculation with the formula:

% Alkaloid = W1+W2–W1

Flavonoid determination

Determination of flavonoid in the samples was achieved by the method of Bohm and Koupai-Abyazani¹⁵. 10g of sample was put in a conical flask of 250ml. Unto this was added 100ml of 80% aqueous methanol, followed by a 3 hours shaking, using an electronic shaker for proper mixing. The mixture was filtered into a previously weighed beaker, and evaporated to dryness over a water bath and weighed to a constant.

Flavonoids in sample was calculated in % with the formula:

Where, W1=weight of empty beaker and W2=weight of residue (weight of empty beaker + sample after drying).

Determination of Saponin

Saponin in both samples was determined using Obadoni and Ochuko method¹⁶. Into a conical flask was 10g of powered bean placed followed by an addition of 100ml of 20% aqueous ethanol. The mixture was thoroughly mixed for about 20 to 30 minutes and was immediately transferred into a conical flask of 250ml. This was covered properly and heated in hot water bath at 90^oC for 4 hours, while stirring for proper mixing. Whatman filter paper was used to filter the resulting mixture. The solid residue was mixed with 100ml of another 20% ethanol and heated in a similar way for 4hours. The solution was then filtered and mixed with the previously filtered solution. The combined filtered solution in a beaker was put on a hot water bath at 90ºC and concentrated until reduced to 20% its initial volume. The resultant solution was removed into a separating funnel of 250ml into which was added 10ml of diethyl ether and properly mixed. Formed layer of diethyl layer was discarded carefully after settling down the solution. This process was repeated and addition of 60ml n-butanol resulted in formation of two layers, the bottom layer was discarded and the upper layer recovered. 10ml of 5% NaCl solution was used to wash the combined n-butanol extract two times. Solvent evaporation was done on the upper layer solution by heating in a water bath at 50⁰C until the solution turned into semi-dried form.

% saponin in sample was calculated by the formula:

Saponin% = (W₂ – W₁) × 100

Where, W1=weight of empty beaker and W2=weight of beaker + sample after drying

Determination of Glycosides

Glycosides contents in samples were determined by the method of Amadi et al.¹⁷. Five gram (5g) of the sample was soaked in 100ml of distilled water in a 250ml conical flask and agitated for 3 hours. The sample was filtered and the total extract was measured. 2ml of extract was put in a test tube and 2ml of 10% DNS reagent was added. This was allowed to boil for 20 minutes in a beaker of boiling water. Test tube was cooled in cold water and the absorbance read at 540nm using UV-Vis Spectrophotometer, DHG-9101.

% glycoside in sample was calculated by the formula:

Tannin Determination in Sample

The content of tannin in bean power was determined by the method described by Amadi et al., with some modification. 0.5g of the sample was put in a conical flask followed by 50ml of distilled water¹⁷. The flask was shaken for 1 hour and filtered. About 5ml of the filtrate was measured into a 50ml volumetric flask and 5ml of 0.1% tannic acid added. The blank was prepared using 5ml of distilled water in a 50ml volumetric flask. The three flasks were incubated for one and half hour at 20ºC using a water bath and the flasks were made up to 50ml mark with distilled water. Concentration was determined at 760nm using UV-Vis Spectrophotomet-er, DHG-9101.

The concentration of tannin was calculated using the formula:

Where, X=concentration of extract; Y=concentration of standard (tannic acid); Z=concentration of blank.

Proximate Analysis

The proximate contents of the bean were done using standard prescription as described by AOAC¹⁹. The proximate compositions determined include;

Moisture Content

Determination of moisture content in powered bean sample was according to the standard description by AOAC¹⁹, with the use of hot air oven. The sample was thoroughly homogenized in a domestic mixer. About 2-10g of homogenized powered bean sample was put in a clean dry petri-dish pre-dried at 98ºC for 60 minutes. Using a hot air oven at 100ºC, the sample was dried by heating for a period of 2 to 3 hours to overnight. Stable weight of the sample was achieved by periodic weighing. Moisture content in sample was calculated in percentage from the difference between the initial sample weight (W_I) and the final sample weight after drying (W_D).

Where, W_I=Initial sample weight

W_D= Final sample weight

Crude Ash Content

Determination of crude ash content was carried out by the standard description by AOAC¹⁹, using muffle furnace. Muffle furnace was used to heat a platinum crucible to 600ºC for 1 hour, cooled in a desiccator and weighed as W₁. Two gram (2g) of the dried sample was weighed (W₂) into a crucible and heated at low flame by keeping on a clay triangle to char the organic matter. The charred material was kept inside the previously set muffle furnace and heat for 6 to 8 hours to greyish white ash and the crucible was cooled in a desiccator and weighed (W₃). Complete ashing was confirmed after the crucible was heated for 30 minutes again, cooled and weighed.

% crude ash in sample was calculated by the formula:

Where, W₁=Weight of crucible, W₂=Weight of dry matter with crucible taken for ashing, W₃=Weight of crucible with ash

Total Protein

Determination of total protein in the samples was by the standard prescription as described by AOAC ¹⁹, with the use of burette. Series of dilution of 0.2ml, 0.4ml, 0.6ml, 0.8ml and 1ml of the working standard was pipetted in different test tubes. Half (0.5ml) and 1ml of the sample extract was pipetted into two other test tubes. The test tubes were made up to 2ml with distilled water along with the blank tubes. Into all test tubes was 3ml of the burette reagent added, mixed properly and incubated at 37ºC for 15minutes. Colour complex was measured with spectrophotomer at 520nm.

Where, OD (test)=optical density of sample; OD (std) = optical density of standard

Crude Fat

Determination of crude fat of in bean samples was by the standard prescription as described by Pearson^20; James²¹, using Soxhlet apparatus. About 5-10g of dry sample recorded as W₁ was placed in a thimble and a cotton plug was kept on top of it and in turn, it was placed in a Soxhlet apparatus. Into a flat bottom flask weighed as W₂ was half volumes of ether added and distilled for 16 hours the apparatus was cooled and the solvent was filtered into a pre-weighed conical flask. The flask of the apparatus was rinsed with small quantities of ether and then added washings to the above flask. The ether was removed by evaporation and the flask was dried with the fat at 80-100ºC, cooled in a dessicator and weighed as W₃.

The percentage of fat content in sample was calculated by the formula:

Where, W1= Weight of dry matter taken for extraction

W2= Weight of flat bottom flask

W3= Weight of flask with fat

Total Carbohydrate

Determination of carbohydrate contents in bean samples were by the standard prescription of AOAC¹⁹ and by the method of nitrogen free extraction (NFE) described by Pearson²⁰. Series of dilution of 0.2ml, 0.4ml, 0.6ml, 0.8ml and 1.0ml, prepared from the working standard were measured into different test tubes. About 0.1ml and 0.2 ml of the sample solution was pipetted into two separate test tubes and each of the test tubes was made up to 1ml with distilled water. A blank test tube was set with distilled water. About 1ml of phenol solution was added to each tube followed by 5ml of 96% sulphuric acid and was mixed very well. After 10minutes, the contents in the test tubes was mixed and placed in a water bath at 25 to 30ºC for 20 minutes and colour change was read at 490nm. The amount of carbohydrate present was calculated using a standard graph.

The percentage of total carbohydrate present was calculated using the formula:

Absorbance corresponding to 0.1ml of the test=X mg of glucose

100ml of the sample solution contains =  ×100 mg of glucose = % of total carbohydrate present

Crude Fiber

Determination of crude fiber of the samples was carried out by the prescription of AOAC¹⁹. Two gram (2g) of the dried sample was boiled with 200ml of sulphuric acid for 30minutes with bumping chips and filtered through muslin and washed with boiling water until washings are no longer acidic. Upon addition of 200ml Sodium hydroxide solution to the residue, the resulting solution was boiled for 30minutes, filtered with muslin cloth and consecutively washed with 25ml of boiling 1.25% H₂SO_4, three 50ml portions of water and 25ml ethanol. Residues were removed and put in a ashing dish weighed as W₁ and was dried for 2 hours at 130±2ºC. The dish was cooled in a dessicator and weighed as W₂. The dish was heated again for 30 minutes at 600±15ºC, cooled in a dessicator and reweighed as W₃.

Thus % crude fiber content in sample was calculated by the formula:

Statistical Analysis

Analyses were performed in triplicate and average values calculated were expressed according to required units. Analysis of variance (ANOVA) with the IBM statistical package for social sciences (SPSS) for Windows version 23, was used to analyze collected data. The Bonferroni post hoc test was used to identify the means that differ significantly at p<0.05. Results are presented as mean±standard deviation.

RESULTS

Results of qualitative phytochemical analysis of aqueous extracts of fresh kidney bean (FKB) and cooked kidney bean (CKB) samples.

The phytochemical qualitative screening (Table 1) of aqueous extracts of both samples showed that alkaloids were very deeply present (+++) in FKB and deeply present (++) in CKB; both frothing and emulsion saponins were present (+) only in FKB; Cyanogenic glycosides were very deeply present (+++) in FKB and deeply present (++) in CKB; Phenols were very deeply present (+++) in both FKB and CKB; Tannins were deeply present (++) in both FKB and CKB; Terpenoids were present (+) in CKB.

Results of quantitative phytochemical analysis of aqueous extracts of fresh kidney bean (FKB) and cooked kidney bean (CKB) samples.

The results of quantitative analysis of CKB and FKB in Table 2 revealed a significant (p<0.05) difference in the following amount of phytochemical in a decreasing order distribution: Alkaloids in FKB (27.17±0.17%)>  CKB (5.8±0.01%); Flavonoids in CKB (18.27±0.24%) >FKB (10.68±0.33%); Glycosides in CKB (1.36±0.01%)>FKB (1.18±0.02%); Saponins in FKB (1.17±0.01%)>CKB (ND) and Tannins in CKB (1.04±0.01%) > FKB (0.56±0.11%).

Results of proximate analysis of fresh kidney bean (FKB) and cooked kidney bean (CKB) samples

The proximate analysis of the bean samples is shown in Table 3 below. The results showed that moisture content of FKB (3.70±0.01%) was >CKB (3.41±0.01%); ash content of CKB (9.94±0.01%) was >FKB (9.72±0.02%); protein content of CKB (82.290%) was>FKB (22.28%); crude fiber of FKB (16.97±0.01%) was >CKB (6.58±0.01%); crude fat of FKB (14.02±0.01%) was >CKB (8.28±0.01%) and carbohydrate content of CKB (87.29±0.01%) was> FKB (82.90±0.01%).

DISCUSSION

Results of phytochemical quantification revealed that alkaloids and saponins in FKB were higher than those in CKB, flavonoids, glycosides and tannins in CKB were higher than those in FKB. While proximate analysis of cooked sample (CKB) showed that protein content, crude ash content and carbohydrate content were higher than those in fresh sample (FKB). While moisture content, crude fiber and crude fat of fresh sample (FKB) were higher than those in cooked sample (CKB) P. vulgaris.

Qualitative and quantitative phytochemical results are shown in (Table 1 and Table 2). The results revealed higher percentages of flavonoids, glycosides and tannins in CKB than FKB and lower percentages of alkaloids and saponins in CKB than FKB. Higher concentrations of flavonoids were found in the extract of cooked bean. This was consistent with Huber et al.²², who reported that cooking increases the concentrations of flavonoids fractions in P. vulgaris especially catechin, quercetin and quercetin-3-glucoside and kaempferol and kaempferol-3-rutinoside. Quercetin was said to be very useful in the deterrence and management of cancer, inhibition of free radical generation by hydrogen peroxide, blockage of histamine generation and involved in the reversal of cognitive insufficiency²³.   On the contrary, Xu and Chang²⁴ assert that cooking and soaking reduce the concentrations flavonoid in P. vulgaris. Cooking boosted the phenolic compounds’ antioxidant ability of beans and increases its nutritive values²². It is reported that flavonoids are the most abundant secondary metabolites in the plant and their presence in beans are appreciated as they can enhance taste, color and flavor in most beans specie when they are well cooked²². Cooking enhanced the concentration of glycosides in the cooked extract than in the fresh. The level of flavonol glycoside in P. vulgaris was reported to be high in cooked P. vulgaris ²⁵. Cyanogenic glycoside is classified as a significant antinutrient factor in plants which must be reduced or destroyed for human and animal food consumption²⁶. The tannin concentration of cooked P. vulgaris is higher than the fresh sample. Like other dark colour legumes, P. vulgaris (red kidney beans) is known to have high content of tannins and phytic acid than white beans²⁷. This could support the high level of tannins in the cooked sample, that even after cooking, the percentage was still high. However, another study reported contrary reduced tannin content in P. vulgaris after cooking²⁸. Tannins are included as antinutrients in legumes which inhibit the digestion of protein but are destroyed by cooking to increase the functions of amylase and protease and better protein digestion²⁹. Dietary tannins, flavonoids and saponins found in P. vulgaris like other plant sources, are known for their medicinal importance in the preparation of herbal formulation as active constituent to take part in cancer prevention, function as antioxidants and possess anti-inflammatory ability^30,31. Cooking reduced the content of alkaloids in cooked extract of P. vulgaris. Different toxic alkaloids are said to be present in beans especially lupines spp., such as pyrrolizidine and piperidine alkaloids³². However on the contrary, Olaofe and Sanni³³ reported that alkaloids applications in medicine are suited for their non toxicity for spectacular physiological functions. The low alkaloid content in the cooked extract of P. vulgaris was in agreement with a previous work reported that after the treatment of soaking and cooking, the alkaloid content of Lupin Bean was significantly reduced³². On the contrary, Alhassan et al., reported high level of alkaloid in Balanites aegyptiaca kernel³⁴.

Proximate analysis of cooked sample (CKB) showed that protein content, crude ash content and carbohydrate content were higher than those in fresh sample (FKB). While moisture content, crude fiber and crude fat of fresh sample (FKB) were higher than those in cooked sample (CKB) P. vulgaris. Cooking was seen to increase the concentration of protein in this study. But in the contrary, Omenna et al.³⁵ reported that heat treatment of pressure cooking and boiling had reductive effect of amino acids including lysine, methionine and tryptophan in Vigna unguiculata and protein was reported to be significantly reduced by boiling in Vigna Sesquipedalis³⁶. In a diet combination experiment, the rich protein content in beans was reported to induce the higher weight loss in an 8 week period among 35 obese men^3,37. Protein consumption in legumes was found to be associated to decrease in blood pressure, total cholesterol, waist circumference and body fat mass³⁷. Similarly, a slightly higher crude ash was found in the cooked extract of P. vulgaris. This finding is in contrast to that reported in a previous study, that boiling reduced the content of ash in beans³⁵. The amount of mineral present in plant is attributed to its ash content, thus increased crude ash could suggest high mineral contents in P. vulgaris extract ³⁶. Higher amount of carbohydrate was found in cooked extract than in fresh extract. This is in agreement with Nzewi and Egbuonu³⁶, who asserted that boiling extensively increased the content of carbohydrate in P. vulgaris 58.70±0.7% to 63.37± 0.11% (8% increase), a value which is well lower than the one reported here, indicating a consequence of cooking on carbohydrate in P. vulgaris.  Carbohydrates contain resistant starch and cooking of legumes in boiling water is reported to decreases the resistant starch content because of destruction of a huge part of the crystalline regions. However, due to a great amount of amylose in legumes and a stable form of crystalline amylase, an infinitesimal quantity of resistant starch is reported to be eliminated by cooking of legumes eventually³⁸. Because the metabolism of resistant starch does not occur in the small intestine, glucose are unconfined into the blood, therefore the need for insulin and food calorific density, which may tend to obesity are reduced³⁹. Furthermore, resistant starch in beans is also reported to increase satiety, reduces glycemic index, lower systolic blood pressure, reduces coronary heart disease risk and prevent rise in blood glucose level⁴⁰. The moisture content of this finding was higher in fresh extract than in cooked extract of P. vulgaris. This is consistent with Nzewi and Egbuonu³⁶, who reported that heat treatment by boiling and roasting significantly reduced the moisture content of V. sesquipedalis but in contrast to Omenna et al.³⁵, who reported that boiling increased the moisture content of Vigna unguiculata. Reduced moisture content in legumes plays a role in food storage and preservation as it guarantees a microbial growth inhibition¹⁰. The moisture content was reduced possibly because of the high temperature subjection which favors evaporation of the cooked extract for the period it was cooked³⁵.  Also, preservation of cooked sample may be enhanced since reduced moisture content is associated with low free fatty acids and acid value⁴¹. Crude fiber of cooked P. vulgaris extract was found to be reduced. This is in agreement to Nzewi and Egbuonu³⁶, who reported that boiling and roasting reduced crude fiber content in V. sesquipedalis were crude fiber was reported to be reduced after boiling³⁵. The work of Nzewi et al. is in agreement with this finding as it was reported that boiling and roasting reduced the fat content as time increases in V. sesquipedalis³⁶. Similarly, Omenna et al.,³⁵ reported reduced content of crude fat after boiling of V. unguiculata. The little or absence of Na, trans–fat, cholesterol and total fat in beans could be contributive to its health stabilizing ability for healthy consumers^42,43. Little or no saturated fat content in cooked beans is recommended for the reduction of cardiovascular risk factors such as raised blood levels of triacylglycerol and low density lipoprotein cholesterol³.

CONCLUSION

Cooking affected the nutrient contents of P. vulgaris in this study. Beans generally are rich sources of both soluble and insoluble fiber which play a very important role in bowel movement function. The presence of fiber in food helps to keep proper functioning of digestive system and provides satiety or fullness.  It was observed that cooking significantly reduced the crude fat. Cooking increased the levels of flavonoids, carbohydrate and protein contents.

CONFLICT OF INTEREST

No conflict of interest exists between authors as it relates this work.

AUTHOR'S CONTRIBUTION

All authors have worked equally in this work.

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Table 1: Results of qualitative phytochemical analysis of FKB and CKB samples

Very deeply present (+++), deeply present (++), present (+), and not detected (ND), Fresh kidney beans (FKB) and Cooked kidney beans (CKB).

Table 2: Results of quantitative phytochemical analysis of RBEB and CBEB samples

Results are Mean ± Standard deviation for duplicate analysis; the mean difference is significant at P<0.05. Fresh Kidney beans (FKB) and cooked kidney beans (CKB).

Table 3: Results of proximate analysis of RBEB and CBEB samples

Results are Mean ± Standard deviation for duplicate analysis; the mean difference is significant at P<0.05. Keywords: Fresh Kidney beans (FKB) and cooked kidney beans (CKB). Fresh kidney beans (FKB) and cooked kidney beans (CKB).