_1568963040 1568963040 Editor editor.jddt@gmail.com UJPR Universal Journal of Pharmaceutical Research Univ J Pharm Res 2456-8058 04 11 2018 Muhammad Shahzad Aslam Rubina Rehman Bashir A. Choudhary Abdul Subhan Ijaz Muhammad Uzair Muhammad Syarhabil Ahmad The present research is preliminary biological screening of aerial plant of Ranunculus muricatus(Ranunculaceae). Dichloromethane and methanol extracts of the aerial plant were investigated for their antifungal, phytotoxic and cytotoxic activities.   Anti-fungal, cytotoxicity and phytotoxicity activities were performed by agar tube dilution assay, brine shrimp lethality bioassay and lemna bioassay respectively. Dichloromethane and methanolic extracts exhibited significant phytotoxicity against Lemna minor having Paraquat as standard drug and incubation condition (28 ± 1ºC). None of extracts presented any significant cytotoxic activity having Imipenum and Etoposide as standard drug respectively. Both extract had non-significant antifungal activity but it has been noted that methanol extract showed 30% inhibition with linear growth at 70 mm, when compared with control; only against Microsporum canis.  Ranunculus muricatus showed significant phytotoxicity. The phytotoxicty assay is a valuable major screen for weedicide investigation. Additionally, modern studies are currently carried out to identify the allelopathic constituents by isolation, purification and structure elucidation to find out as effective herbicidal. Google Scholar 09 01 2016 10.22270/ujpr.v1i1.R2 http://ujpr.org/index.php/journal/article/view/12 http://ujpr.org/index.php/journal/article/download/12/8 http://ujpr.org/index.php/journal/article/download/12/191 http://ujpr.org/index.php/journal/article/download/12/8 http://ujpr.org/index.php/journal/article/download/12/191