_1736920621 20250115055701000 Editor editor.jddt@gmail.com Universal Journal of Pharmaceutical Research Universal Journal of Pharmaceutical Research Univ J Pharm Res 2456-8058 2831-5235 01 15 2025 Rahmawati Muzakkir Baits https://orcid.org/0000-0003-2465-9953 Tadjuddin Naid https://orcid.org/0009-0002-9755-4757 Nurul Azmi https://orcid.org/0009-0005-1241-0503 Raudhia Wulandari Rumata https://orcid.org/0009-0005-1241-0503 Aims and objectives: To ascertain the antibacterial capability using TLC-bioautography and the tannin content using the UV-Vis spectrophotometric method in the ethanol extract of kopasanda (Chromolaena odorata L.) leaves. Methods: Tannins quantitatively utilizing the UV-Vis spectrophotometric technique using the folin ciocalteu reagent at a wavelength of up to 687 nm. Antibacterial potential against gastrointestinal infection-causing bacteria (Salmonella typhi, Vibrio cholerae, Escherichia coli, and Shigella dysentriae) is assessed using TLC-bioautography technique. Results: The tannin content of the ethanol extract of kopasanda leaves (Chromolaena odorata L.) is 41.9064±0.26 mgTAE/g extract. Escherichia coli, Shigella dysentriae, Salmonella typhi, and Vibrio cholerae, respectively, create inhibitory zones in the antibacterial potential test that yields 7, 8, 8, and 7 stains. Tannins, flavonoids, alkaloids, and saponins are believed to be among the chemicals found in the TLC results obtained with stain spraying reagents 1, 3, 5, and 4, respectively. Conclusions: The average tannin concentration of the ethanol extract of kopasanda leaves (Chromolaena odorata L.) is 41.9064±0.26 mgTAE/g extract, and it has antibacterial properties against germs that cause gastrointestinal illnesses.                   Peer Review History: Received 22 September 2024;   Reviewed 3 November; Accepted 15 December; Available online 15 January 2025 Academic Editor: Dr. Amany Mohamed Alboghdadly, Ibn Sina National College for Medical Studies in Jeddah, Saudi Arabia, amanyalboghdadly@gmail.com  Average Peer review marks at initial stage: 6.0/10 Average Peer review marks at publication stage: 7.0/10 01 15 2025 http://creativecommons.org/licenses/by-nc/4.0 10.22270/ujpr.v9i6.1235 https://ujpronline.com/index.php/journal/article/view/1235 https://ujpronline.com/index.php/journal/article/download/1235/1760 https://ujpronline.com/index.php/journal/article/download/1235/1760 https://ujpronline.com/index.php/journal/article/download/1235/1773 https://ujpronline.com/index.php/journal/article/download/1235/1788