_1747381208 20250516074008000 Editor editor.jddt@gmail.com Universal Journal of Pharmaceutical Research Universal Journal of Pharmaceutical Research Univ J Pharm Res 2456-8058 2831-5235 05 15 2025 Agustina L Lailiyah M Yuliati N Prasongko ET Istiqomah Soehartono DR Dayoob M Background and aims: Acne develops due to the obstruction of pilosebaceous follicles by accumulated sebum, keratinocytes, and microbial colonization Certain secondary metabolites from plants possess anti-acne properties, including karsen (Muntingia calabura L.). This study aims to formulate and evaluate the anti-acne activity of an emulgel against Propionibacterium acnes. The ethanolic extract from Muntingia calabura L. leaves was used in the formulation. Methods: The extraction process was carried out using maceration. The ethanolic extract of karsen was utilized as the active ingredient in the emulgel formulation. The emulgel was evaluated based on its organoleptic properties, homogeneity, pH, emulsion type, spreadability, and adhesion. The antibacterial activity was assessed through an in vitro antibacterial assay. Results: The extraction yield was 10.8%. The extract contained flavonoids, tannins, and saponins. The formulated emulgel had a semi-solid consistency, a brownish-green color, a distinct odor characteristic of Muntingia calabura L. leaves, and was homogeneous. It had a pH range of 4.87–5.40, an oil-in-water (O/W) emulsion type, a spreadability of 5.1–5.8 cm, and an adhesion time of 3.26–4.36 seconds. The formulation exhibited anti-acne activity against Propionibacterium acnes, with the highest inhibitory activity observed at a concentration of 12%. Conclusions: The ethanolic extract from M. calabura L. leaves can be successfully formulated into a stable emulgel. The formulated emulgel exhibits antibacterial activity against Propionibacterium acnes.                   Peer Review History: Received 3 February 2025;   Reviewed 9 March 2025; Accepted 22 April; Available online 15 May 2025 05 15 2025 http://creativecommons.org/licenses/by-nc/4.0 10.22270/ujpr.v10i2.1304 https://ujpronline.com/index.php/journal/article/view/1304 https://ujpronline.com/index.php/journal/article/download/1304/1847 https://ujpronline.com/index.php/journal/article/download/1304/1847 https://ujpronline.com/index.php/journal/article/download/1304/1861 https://ujpronline.com/index.php/journal/article/download/1304/1875