_1757918392 20250915063952000 Editor editor.jddt@gmail.com Universal Journal of Pharmaceutical Research Universal Journal of Pharmaceutical Research Univ J Pharm Res 2456-8058 2831-5235 09 15 2025 Prince Ogochukwu Alaebo Anthony Cemaluk Chinedum Egbuonu Precious Uchechi Okpara Juliet Chinonso Njoku Chimaobi Anthony Nwede Chidera Nnamdi Abalihe Thankgod Chidumije Ugboaja Livinus Chinonso Chukwu Enyinna Wisdom Chukwuka John Chiemelie Uchegbusi Kenneth Ifechukwu Ataka Background and aim: Oxidative stress is a major factor in both the progression and the adverse effects of diabetes mellitus. Natural Bioactive substances like curcumin (derived from turmeric) and gingerol (from ginger) possess antioxidant properties that may offer therapeutic benefits by enhancing the body's defense mechanisms against oxidative damage. This study investigates the synergistic antioxidant effects of co-administered curcumin and gingerol in managing oxidative stress associated with diabetes mellitus. Method: Oxidative stress biomarkers including superoxide dismutase (SOD), malondialdehyde (MDA), catalase, and glutathione were evaluated in experimental rat groups treated with varying doses of curcumin and gingerol. Additionally, in vitro antioxidant activities of turmeric and ginger extracts were assessed using DPPH and FRAP assays. Results: The combination of 400 mg/kg curcumin and gingerol significantly increased SOD (24.49±6.85 IU/g protein) and catalase (19.02±1.76 kU/g protein) activities (p<0.05) compared to other groups. Glutathione levels also rose notably (5.31±1.26 µg/L), indicating enhanced endogenous antioxidant capacity. Although MDA levels (75.05±24.76 nmol/g protein) were higher than in lower dose groups, they remained lower than in the 200 mg/kg group (135.35±8.51 nmol/g protein), suggesting a dose dependent response. In vitro assays confirmed strong radical scavenging and reducing activities, with turmeric slightly outperforming in FRAP, while ginger matched in DPPH scavenging. Conclusion: Co-administration of curcumin and gingerol enhances antioxidant defenses more effectively than individual treatments or metformin. This synergy presents a potential adjunct therapy for mitigating oxidative damage in diabetes and merits further clinical investigation.                     Peer Review History: Received 7 June 2025;   Reviewed 11 July 2025; Accepted 18 August; Available online 15 September 2025 Academic Editor: Dr. Amany Mohamed Alboghdadly, Ibn Sina National College for Medical Studies in Jeddah, Saudi Arabia, amanyalboghdadly@gmail.com  Reviewers: Antonio José de Jesus Evangelista, Federal University of Ceará, UFC, Brazil, tony_biomed@hotmail.com Dr. Ahmad Abdelsattar El-Ebiary, Tanta University Hospitals, Tanta, Egypt, a.ebiary@med.tanta.edu.eg 09 15 2025 http://creativecommons.org/licenses/by-nc/4.0 10.22270/ujpr.v10i4.1389 https://ujpronline.com/index.php/journal/article/view/1389 https://ujpronline.com/index.php/journal/article/download/1389/1940 https://ujpronline.com/index.php/journal/article/download/1389/1940 https://ujpronline.com/index.php/journal/article/download/1389/1954 https://ujpronline.com/index.php/journal/article/download/1389/1970