_1763201627 20251115101347000 Editor editor.jddt@gmail.com Universal Journal of Pharmaceutical Research Universal Journal of Pharmaceutical Research Univ J Pharm Res 2456-8058 2831-5235 11 15 2025 Robert Tungadi Teti Sutriyati Tuloli Jenifer Sakul Background: Functional probiotic beverages derived from plant matrices offer sustainable alternatives to dairy-based products but require technological strategies to maintain probiotic viability. Objective: This study aimed to formulate, characterize, and assess the probiotic viability, release profile, and stability of corn milk nanoparticles prepared by ionic gelation. Methods: Sodium alginate and calcium chloride were employed in an 8:1 ratio to form nanoparticles containing Lactobacillus shirota. Characterization included particle size, morphology (SEM), in vitro release, and stability testing. Results: The nanoparticles exhibited a mean diameter of 447.6 nm (PDI = 0.454) with a smooth spherical morphology. Controlled release assays demonstrated a cumulative release of 86.2±2.5% viable probiotics after 4 h in simulated intestinal fluid, confirming sustained delivery potential. Stability assessment showed retention of >80% viable cells after 30 days at 4 °C. Conclusion: Corn milk nanoparticles produced by ionic gelation exhibited favorable size, morphology, release behavior, and stability, confirming their potential as a non-dairy probiotic delivery system.                     Peer Review History: Received 3 August 2025;   Reviewed 11 September 2025; Accepted 17 October; Available online 15 November 2025 Academic Editor: Dr. DANIYAN Oluwatoyin Michael, Obafemi Awolowo University, ILE-IFE, Nigeria, toyinpharm@gmail.com Reviewers: Dr. Jennifer Audu-Peter, University of Jos, Nigeria, drambia44@gmail.com Dr. Kamal Elbssir Mohammed Ali, Hail University KSA, kamalelbssir999@gmail.com 11 15 2025 http://creativecommons.org/licenses/by-nc/4.0 10.22270/ujpr.v10i5.1422 https://ujpronline.com/index.php/journal/article/view/1422 https://ujpronline.com/index.php/journal/article/download/1422/1988 https://ujpronline.com/index.php/journal/article/download/1422/1988 https://ujpronline.com/index.php/journal/article/download/1422/2001 https://ujpronline.com/index.php/journal/article/download/1422/2016