_1763201677 20251115101437000 Editor editor.jddt@gmail.com Universal Journal of Pharmaceutical Research Universal Journal of Pharmaceutical Research Univ J Pharm Res 2456-8058 2831-5235 11 15 2025 Mostafa Essam Eissa Background and aims: Healthcare associated infections are a major concern, causing an estimated 72,000 deaths and costing the United States up to $45 billion annually. This study aimed to identify and characterize bacterial isolates in healthcare facilities to improve infection control. Methods: Using biochemical identification and Pareto analysis, samples were examined to determine the most prevalent bacterial species. The Pareto principle helped focus efforts on the major contaminants. Results: Results showed that Pseudomonas genus (34.6%) and Micrococcus genus (19.2%) were the most abundant, accounting for more than 50% of isolates. The presence of multiple bacterial species, including both Gram-positive and Gram-negative bacteria, suggests widespread prevalence, likely a result of inadequate cleaning and contamination of water or surfaces. Gram-positive bacilli were less common due to their lower environmental resistance. Conclusions: The study concluded that implementing proper cleaning and disinfection protocols and regularly monitoring water quality are essential for preventing cross-infection and ensuring a safe environment. Identifying the most prevalent bacteria using the Pareto principle is a crucial step in mitigating the risk of microbial contamination.                   Peer Review History: Received 8 August 2025;   Reviewed 11 September 2025; Accepted 19 October; Available online 15 November 2025 Academic Editor: Dr. Emmanuel O. Olorunsola, Department of Pharmaceutics & Pharmaceutical Technology, University of Uyo, Nigeria, olorunsolaeo@yahoo.com Reviewers: Prof. Hassan A.H. Al-Shamahy, Sana'a University, Yemen, shmahe@yemen.net.ye  Dr. Mohaddese Mahboubi, Alzahra University, Tehran, Iran, mahboubi@barijessence.com 11 15 2025 http://creativecommons.org/licenses/by-nc/4.0 10.22270/ujpr.v10i5.1424 https://ujpronline.com/index.php/journal/article/view/1424 https://ujpronline.com/index.php/journal/article/download/1424/1990 https://ujpronline.com/index.php/journal/article/download/1424/1990 https://ujpronline.com/index.php/journal/article/download/1424/2003 https://ujpronline.com/index.php/journal/article/download/1424/2018