_1568707118 1568707118 Editor editor.jddt@gmail.com UJPR Universal Journal of Pharmaceutical Research Univ J Pharm Res 2456-8058 09 15 2019 Ahmet Ozbilgin Husniye Kayalar Ibrahim Cavus Cenk Durmuskahya Umit Toktas Cumhur Gunduz In the present study it was aimed to determine the in vitro antileishmanial activity of Berberis vulgaris L. against Leishmania  tropica promastigotes. The aerial parts of Berberis vulgaris were collected from Spil Mountain, Manisa. The ethanolic extract of the plant material was prepared. The consecutive concentrations of the plant extract (25-100µg/ml) were set for in vitro antileishmanial assays. In addition to in vitro inhibitory activities against Leishmania tropica promastigotes, the cytotoxicity of the plant extract was also measured by WST-1 Cell proliferation assay. The percentages of parasite inhibition in the presence of B. vulgaris ethanol extract in comparison with glucantime reference group at time interval of 12-72 hours were observed between 88.0 and 100.0 %. The plant extract was found to have cytotoxic activity with 444, 81±2, 12 µg/ml IC50 value.This is the first study that involves the in vitro antileishmanial activity of B. vulgaris which is wildly growing in Manisa, Turkey. Initial results demonstrated that the ethanolic extract of B. vulgaris gave promising results and it could be used as an antileishmanial agent in future. 09 12 2019 10.22270/ujpr.v4i4.294 http://ujpr.org/index.php/journal/article/view/294 http://ujpr.org/index.php/journal/article/download/294/365 http://ujpr.org/index.php/journal/article/download/294/377 http://ujpr.org/index.php/journal/article/download/294/365 http://ujpr.org/index.php/journal/article/download/294/377