_1647319505 1647319505 Editor editor.jddt@gmail.com Universal Journal of Pharmaceutical Research Universal Journal of Pharmaceutical Research Univ J Pharm Res 2456-8058 03 15 2022 Dyah Ratna Ayu Puspita Sari https://orcid.org/0000-0003-3456-0260 Putu Yudha Ugrasena https://orcid.org/0000-0002-1069-4961 Ni Wayan Ria Ekayani https://orcid.org/0000-0002-5287-9024 Aim and objective: Acanthus ilicifolius also known as jeruju is a plant that has a lot of bioactivity that can be used as a potential medicinal plant development. This study aimed to determine the cytotoxicity activity of ethanol extract of A. ilicifolius leaves. Methods: This study used Brine Shrimp Lethality Test (BSLT) method using Artemia salina larvae with test solution series concentration of 10, 50, 100, 500, 1000 ppm and control without extract. A. salina was added to each test tube. After 24 hours, the larvae mortality was observed. LC50 assessment was analyzedwith probit analysisby  Microsoft excel. Result: The largest mortality percentage was shown at a concentration of 1000 ppm ethanol extract of A. ilicifolius leaves with an average mortality value of 80%, while the concentrations of 10 ppm, 50 ppm, 100 ppm, and 500 ppm had an average mortality value of 20%, 40%, 57% and 63%.The ethanol extract of A. ilicifolius leaves categorized as toxic with a LC50 value of 103,6 ppm. Conclusion: It was concluded that ethanol extract of A. ilicifolius leaves has cytotoxicity activity potential.                           Peer Review History: Received: 11 January 2022; Revised: 12 February; Accepted: 3 March, Available online: 15 March 2022 Academic Editor: Dr. Gehan Fawzy Abdel Raoof Kandeel, Pharmacognosy Department, National Research Centre, Dokki, 12622,  Giza, Egypt, gehankandeel9@yahoo.com  UJPR follows the most transparent and toughest ‘Advanced OPEN peer review’ system. The identity of the authors and, reviewers will be known to each other. This transparent process will help to eradicate any possible malicious/purposeful interference by any person (publishing staff, reviewer, editor, author, etc) during peer review. As a result of this unique system, all reviewers will get their due recognition and respect, once their names are published in the papers. We expect that, by publishing peer review reports with published papers, will be helpful to many authors for drafting their article according to the specifications. Auhors will remove any error of their article and they will improve their article(s) according to the previous reports displayed with published article(s). The main purpose of it is ‘to improve the quality of a candidate manuscript’. Our reviewers check the ‘strength and weakness of a manuscript honestly’. There will increase in the perfection, and transparency.  Received file:                Reviewer's Comments: Average Peer review marks at initial stage: 5.5/10 Average Peer review marks at publication stage: 7.0/10 Reviewers: Ahmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, ahmad.najib@umi.ac.id Dr. A.A. Mgbahurike, University of Port Harcourt, Nigeria, amaka_mgbahurike@yahoo.com Similar Articles: ANTIFUNGAL, CYTOTOXIC AND PHYTOTOXICITY OF AERIAL PART OF RANUNCULUS MURICATUS CYTOTOXIC EFFECT AND PHYTOCHEMICAL STUDY OF PETROLEUM ETHER EXTRACT OF TILIA CORDATA MILL 03 15 2022 10.22270/ujpr.v7i1.724 http://ujpr.org/test/index.php/journal/article/view/724 http://ujpr.org/test/index.php/journal/article/download/724/1135 http://ujpr.org/test/index.php/journal/article/download/724/1146 http://ujpr.org/test/index.php/journal/article/download/724/1158 http://ujpr.org/test/index.php/journal/article/download/724/1135 http://ujpr.org/test/index.php/journal/article/download/724/1146 http://ujpr.org/test/index.php/journal/article/download/724/1158