MODULATION OF MITOCHONDRIAL MEDIATED APOPTOSIS BY SOLVENT FRACTIONS OF THE FRUIT EXTRACTS OF SARCOCEPHALUS LATIFOLIUS (SMITH) BRUCE

  • Joan Uchechukwu Imah-Harry Department of Natural Sciences, Faculty of Pure and Applied Sciences, Precious Cornerstone University, Ibadan, Nigeria.
  • Olufunso Olabode Olorunsogo Laboratories for Biomembrane Research and Biotechnology Department of Biochemistry, College of Medicine, University of Ibadan, Nigeria.
10.22270/ujpr.v9i5.1205

Keywords:

Apoptosis, cancer, MOMP, mPT pore, Sarcocephalus latifolius

Abstract

Aim and Objective: However, this claim has not been scientifically substantiated. In this study, we investigated the inductive effect of the crude methanol extract (CMESL) and a chloroform subfraction (sCFSL) of Sarcocephalus latifolius fruits on mPT, in vivo

Methods: Thirty-five male wistar rats (90±10 g) were acclimatized, divided into seven groups, and treated with 1% DMSO (control) and 25, 50, and 100 mg/kgbw of each of the fractions for thirty days. Rats were sacrificed and liver mitochondria were isolated by differential centrifugation.  The MOMP, DNA fragmentation, p53, Bax and BCl-2 protein expressions, Cytochrome C release, and caspase – 3 and -9 activities were assayed in liver tissue by standard methods. 

Results: CMESL and sCFSL induced mPT pore. The sCFSL induced MOMP maximally at 100 mg/kgbw, inhibited LPO (78%), enhanced mitochondrial ATPase activity (27.96±0.04 µmole/mg Protein/min) than CMESL (17.58± 0.03 µmole/mg Protein/min) at the same dose. Similarly, sCFSL caused DNA fragmentation; (77.33%), enhanced caspases -3 and -9 activation; increased p53 and Bax expression levels, increased Cytochrome C release, and downregulated BCl-2 protein expression, compared to CMESL. 

Conclusion: These findings showed that sCFSL contains bioactive agents that can induce mitochondrial-mediated apoptosis, and therefore a potential target to be explored in the management of tumors and cancer. 

                  

Peer Review History:

Received 12 July 2024;   Reviewed 19 September 2024; Accepted 24 October; Available online 15 November 2024

Academic Editor: Dr. Iman Muhammad Higazyorcid22.jpg, National Research Center, Egypt, imane.higazy@hotmail.com

Average Peer review marks at initial stage: 6.5/10

Average Peer review marks at publication stage: 7.5/10

Reviewers:

orcid22.jpgDr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my

orcid22.jpgDr. Nazim Hussain, North East Frontier Technical University, Arunachal pradesh, India, nhussain116@gmail.com

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Published

2024-11-15

How to Cite

Imah-Harry, J. U., and O. O. Olorunsogo. “MODULATION OF MITOCHONDRIAL MEDIATED APOPTOSIS BY SOLVENT FRACTIONS OF THE FRUIT EXTRACTS OF SARCOCEPHALUS LATIFOLIUS (SMITH) BRUCE”. Universal Journal of Pharmaceutical Research, vol. 9, no. 5, Nov. 2024, doi:10.22270/ujpr.v9i5.1205.

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