UNVEILING THE CULTURABLE BACILLUS SPP. ISOLATED FROM HONEY IN BURKINA FASO AS A POTENTIAL SOURCE FOR ANTIMICROBIAL PEPTIDES

Sonagnon H.S. Kouhounde; Ulysse Daa Kpode; Hilarie Bonane; Rene Comlan Yaovi; Kifouli Adeoti; Mamoudou H. Dicko

doi:10.22270/ujpr.v11i3.1585

Sonagnon H.S. Kouhounde Laboratory of Applied Biological Sciences, New Dawn University, 01 BP 234 Bobo-Dioulasso 01, Burkina Faso.
Ulysse Daa Kpode Laboratory of Food Microbiology Technology and Phytopathology, University of Abomey-Calavi, Benin. Team of Plant Biodiversity and Ecology, University of Abomey-Calavi, Benin.
Hilarie Bonane Laboratory of Applied Biological Sciences, New Dawn University, 01 BP 234 Bobo-Dioulasso 01, Burkina Faso.
Rene Comlan Yaovi Laboratory of Agrosystems Bioresources and Environmental Health, Nazi Boni University, Bobo-Dioulasso, Burkina Faso
Kifouli Adeoti Laboratory of Food Microbiology Technology and Phytopathology, University of Abomey-Calavi, Benin. Team of Plant Biodiversity and Ecology, University of Abomey-Calavi, Benin.
Mamoudou H. Dicko Laboratory of Biochemistry, Biotechnology, Food Technology, and Nutrition, Joseph KI-ZERBO University, 03 BP 7021 Ouagadougou, Burkina Faso.

10.22270/ujpr.v11i3.1585

Keywords:

Antimicrobial peptides, AMP-genes, Bacillus spp., Burkina Faso, Honey microbiota

Abstract

Backgrounds and objectives: Honey is increasingly recognized as a promising reservoir of beneficial microorganisms with activity against plant pathogens. However it is poorly documented that in the literature. This study contributes to explore the diversity of AMPs genes produced by Bacillus spp. isolated from honey in Burkina Faso in order to propose a biological solution against biodiversity loss, water soil pollution and pest resistance caused by chemicals pesticides.

Methods: Methodology was based on isolation and preliminarily characterization of 40 presumptive Bacillus spp. followed by their survival under thermal stress, salt tolerance and AMPs related genes identification.

Results: Data from PCR analysis revealed that all isolates belonged to the genus Bacillus. In total, 70% of strains carried at least one AMP gene, in which 50% carried one gene. Regarding AMPs-gene associations, 25% carried two genes, 14.3% carried three genes, 7.1% carried four genes, and 3.6% carried five of the six targeted AMP-genes.

Conclusions: These data indicate that honey produced in Burkina Faso could be used to isolate Bacillus strains with genetic potential for broad-spectrum antimicrobial activity. This efficacy could be tested in future studies to confirm these findings.