IN-VITRO ANTIBACTERIAL ACTIVITY OF LACTOBACILLI METABOLITES LOADED HYDROGEL FORMULATIONS AGAINST PSEUDOMONAS AERUGINOSA

Abstract

Objective: Pseudomonas aeruginosa is an opportunistic pathogen that may cause serious infections, ranging from localized skin infections to life-threatening systemic diseases. It is one of the resistant bacteria which lead to failure in the treatment. New treatment strategies are urgently needed for the treatment of infections caused by resistant bacteria. Researchers are interested in Lactobacillus species because of their antimicrobial effective metabolites. The aim of this study was comparing the antibacterial activity of lactobacilli metabolites loaded chitosan and Pluronic F127 hydrogel formulations.
Methods: Metabolites loaded hydrogel formulations were prepared by adding 20 µl of supernatant into the polymer solutions. The antimicrobial activities of two hydrogel formulations were investigated against P. aeruginosa using Agar Spot Method. Metabolites loaded chitosan hydrogel formulations were found to be effective against P. aeruginosa.
Results: No antibacterial activity was observed for metabolites loaded Pluronic F127 hydrogel formulations. When we compare these two hydrogel formulations, we observed that hydrogel formulation prepared by using chitosan released effective metabolites but Pluronic F127 hydrogel formulation couldn’t release antibacterial effective metabolites.
Conclusion: The antibacterial effect of metabolites loaded chitosan hydrogel formulation suggests that this formulation could be used as an alternative treatment option in P. aeruginosa skin infections. However, further investigations are needed to develop a pharmaceutical product.

             
Peer Review History:

Received 7 June 2019;   Revised 12 July; Accepted 29 August; Available online 15 September 2019

Academic Editor: Rola Jadallah, Arab American University, Palestine, rola@aauj.edu

Received file:                Reviewer's Comments:

Average Peer review marks at initial stage: 7/10

Average Peer review marks at publication stage: 9.4/10

Reviewer(s) detail:

Prof. Dr. Gorkem Dulger, Duzce University, Turkey, gorkemdulger@yandex.com

Prof. Dr. Hassan A.H. Al-Shamahy, Sana'a University, Yemen, shmahe@yemen.net.ye