ANTIOXIDANT, ANTI-INFLAMMATORY, ANTIDIABETIC, ANTHELMINTHIC, AND THROMBOLYTIC ACTIVITY OF THE METHANOL EXTRACT OF CITRULLUS LANATUS PEEL

Nazmun Nahar; Ummul Khair Fatema; Partha Protim Sharma; Abdul Motaleb Bhuiya; Taslima Begum; Nur Fauwizah Azahar; Mohammad Nasrin Abdul Rahman; Wan Mohd Azizi Bin Wan Sulaiman; Abul Kalam Azad

doi:10.22270/ujpr.v9i2.1085

Nazmun Nahar Department of Pharmacy, Basic medical and Pharmaceutical Sciences, USTC, Chittagong, Bangladesh.
Ummul Khair Fatema Department of Pharmacy, Basic medical and Pharmaceutical Sciences, USTC, Chittagong, Bangladesh.
Partha Protim Sharma Department of Pharmacy, Basic medical and Pharmaceutical Sciences, USTC, Chittagong, Bangladesh.
Abdul Motaleb Bhuiya Department of Pharmacy, Basic medical and Pharmaceutical Sciences, USTC, Chittagong, Bangladesh.
Taslima Begum Department of Pharmaceutical Chemistry, Faculty of Pharmacy, International Islamic University Malaysia, 25200 Kuantan, Pahang, Malaysia.
Nur Fauwizah Azahar Centre for Pre-University Studies, MAHSA University, Bandar Saujana Putra, 42610 Jenjarom, Selangor, Malaysia.
Mohammad Nasrin Abdul Rahman Department of Pharmaceutical Technology, Faculty of Pharmacy, University College MAIWP International, 68100 Kuala Lumpur, Malaysia.
Wan Mohd Azizi Bin Wan Sulaiman Department of Pharmaceutical Technology, Faculty of Pharmacy, University College MAIWP International, 68100 Kuala Lumpur, Malaysia.
Abul Kalam Azad Department of Pharmaceutical Technology, Faculty of Pharmacy, University College MAIWP International, 68100 Kuala Lumpur, Malaysia.

10.22270/ujpr.v9i2.1085

Keywords:

Anthelmintic, antioxidative, anti-inflammatory, antidiabetic, CLP, thrombolytic

Abstract

Background: The present study aimed for in vitro evaluation of Citrullus lanatus Peel (CLP) methanol extract for antioxidative, anti-inflammatory, antidiabetic, anthelmintic, and thrombolytic activity.

Method: CLP was extracted with methanol and tested for in vitro antioxidative (DPPH and H₂O₂scavenging method), thrombolytic, anthelmintic, antidiabetic, and anti-inflammatory properties (human red blood cell membrane stabilization method, α-amylase inhibitory effect by using Iodine-Starch method.

Results: The percentage yield was 7% and phytochemical profile showed the presence of steroid, tannins, saponins, flavonoids, glycoside and reducing sugar. In DPPH scavenging assay, 88.7% was at 1000 g/ml, whereas 34% to 48.9% (IC₅₀27.65 µg/ml) in H₂O₂and from 61.8% to 95.2% (IC₅₀ value: 07 µg/ml) in ASCOR. In in vitro anti-inflammatory activity, the maximum protection percentage was observed 58% and 44%. Consecutively, in anti-diabetic activity, IC₅₀ values of 1137.64 μg/ml whereas reference (Acarbose) exhibited IC₅₀at 671.71 μg/ml. On the other hand, the inhibitory effect of Acarbose showed 57% and 98% respectively at 2000 μg/ml. In earthworms, it considered as very potential for the helminthic infection and helminthiasis. A significant clot lysis activity of 44% was noticed when the clots were treated with 100 µl of CLP extract (10 mg/ml) in in-vitro thrombolytic activity assay.

Conclusions: The findings of this study revealed that the peel possessed the antioxidant, anti-inflammatory, antidiabetic, anthelminthic and thrombolytic activities. These findings will be motivated to do further research on molecular level to finds into more depth which is considered as the limitation of this study.