ANTI-INFLAMMATORY EFFECTS OF ROOT BARK METHANOLIC EXTRACT OF BOSWELLIA DALZIELII IN ALBINO MICE

Jeweldai Vedekoi; Elizé Dadaya; Koubé Juliette; Sokeng Dongmo Sélestin

doi:10.22270/ujpr.v9i4.1152

Jeweldai Vedekoi Department of Biotchnologies and Pharmacognosy, Faculty of Science, University of Ebolowa, Ebolowa, Cameroon. Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon.
Elizé Dadaya Department of Life Sciences, Higher Teachers Training College, University of Bertoua, Bertoua, Cameroon.
Koubé Juliette Department of pharmaceutical sciences, Faculty of Medicine and Pharmaceutical Sciences, University of Douala, Cameroon.
Sokeng Dongmo Sélestin Department of Biological Sciences, Faculty of Science, University of Ngaoundere, Ngaoundere, Cameroon.

10.22270/ujpr.v9i4.1152

Keywords:

Anti-inflammatory, B. dalzielii, Carrageenan, DPPH, FRAP, IC50, LD50

Abstract

Background and objective: Boswellia dalzielii has been traditionally used in the Far North region (Cameroon) to treat fever, rheumatism, asthma and various inﬂammatory conditions. This investigation was designed to determine the anti-edematous and antioxidant properties to confirm its storied claims.

Methods: The DPPH radical scavenging and reduced Fe³⁺to the Fe²⁺ assays were two methods used to evaluate the antioxidant activities. The swelling caused by carrageenan paw and xylene ear edema to cause inflammation were applied to evaluate the anti-inflammatory activities of the extract. The mechanisms of action of the methanol extract were evaluated in the serotonin test. Fever induced by yeast was exploited to value antipyretic activity in albinos animals. Diclofenac, ascorbic acid, and paracetamol were utilized as standard medicaments.

Results: Results published the existence of cardiac glycosides, flavonoid, simple phenols, flavonoids, tannins, coumarins and alkaloids. The results indicated also no acute toxicity and the LD₅₀ was above 3000 mg/kg. The methanolic extract exhibited the best activity IC₅₀ (178.12 µg/mL) against DPPH radical with, which was comparable with ascorbic acid (179.12 µg/mL) and a strong reducing power with an IC₅₀ of about 147.85 µg /mL. In addition, the roots extract of B. dalzielii significantly inhibited (p<0.001) the volume of paw edema, ear edema and the fever.

Conclusion: Finally, the present study published clearly that the B. dalzielii methanolic extract (BDME) possess important antioxidant and anti-edematous effects. The phytoconstituents found in this methanol extract were responsible for the anti-inflammatory and antioxidant effects.

Peer Review History:

Received 21 May 2024; Reviewed 15 July 2024; Accepted 25 August; Available online 15 September 2024

Academic Editor: Dr. Gehan Fawzy Abdel Raoof Kandeel, Pharmacognosy Department, National Research Centre, Dokki, 12622, Giza, Egypt, gehankandeel9@yahoo.com