ANTIFUNGAL, CYTOTOXIC AND PHYTOTOXICITY OF AERIAL PART OF RANUNCULUS MURICATUS

Muhammad Shahzad Aslam; Rubina Rehman; Bashir A. Choudhary; Abdul Subhan Ijaz; Muhammad Uzair; Muhammad Syarhabil Ahmad

doi:10.22270/ujpr.v1i1.R2

Muhammad Shahzad Aslam School of Bioprocess Engineering, University Malaysia Perlis, Kompleks Pusat Pengajian, Jejawi 3, 02600 Arau, Perlis, Malaysia.
Rubina Rehman Department of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
Bashir A. Choudhary Department of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
Abdul Subhan Ijaz Department of Pharmacy, Bahauddin Zakariya University, Multan, Pakistan.
Muhammad Uzair School of Bioprocess Engineering, University Malaysia Perlis, Kompleks Pusat Pengajian, Jejawi 3, 02600 Arau, Perlis, Malaysia.
Muhammad Syarhabil Ahmad School of Bioprocess Engineering, University Malaysia Perlis, Kompleks Pusat Pengajian, Jejawi 3, 02600 Arau, Perlis, Malaysia.

10.22270/ujpr.v1i1.R2

Keywords:

Ranunculus muricatus, biological screening, phytotoxicity, cytotoxicity

Abstract

Objectives: The present research is preliminary biological screening of aerial plant of Ranunculus muricatus (Ranunculaceae). Dichloromethane and methanol extracts of the aerial plant were investigated for their antifungal, phytotoxic and cytotoxic activities.

Methods: Anti-fungal, cytotoxicity and phytotoxicity activities were performed by agar tube dilution assay, brine shrimp lethality bioassay and lemna bioassay respectively. Dichloromethane and methanolic extracts exhibited significant phytotoxicity against Lemna minor having Paraquat as standard drug and incubation condition (28±1ºC).

Results: None of extracts presented any significant cytotoxic activity having Imipenum and Etoposide as standard drug respectively. Both extract had non-significant antifungal activity but it has been noted that methanol extract showed 30% inhibition with linear growth at 70 mm, when compared with control; only against Microsporum canis. Ranunculus muricatus showed significant phytotoxicity.

Conclusion: The phytotoxicty assay is a valuable major screen for weedicide investigation. Additionally, modern studies are currently carried out to identify the allelopathic constituents by isolation, purification and structure elucidation to find out as effective herbicidal.