GC-MS ANALYSIS OF FIXED OILS OF NIGELLA SATIVA SEEDS

Abstract

Objective: Gas chromatography-Mass spectrometry (GC-MS) is an analytical method that combines the features of gas-liquid chromatography and mass spectrometry to identify different substances within a test sample. Applications of GC-MS include drug detection, fire investigation, environmental analysis, explosives investigation, and identification of unknown samples.

Methods: The present study also relies on use of GC-MS for detection and interpretation of compounds present in N. sativa oil samples. Fixed oil was obtained through column chromatography of ethyl acetate fraction. The oil samples were subjected to GC-MS analysis which showed 5, 18, 12 and 20 compounds in four fixed oil samples respectively.

Results: The major components were linoleic acid, methyl ester (35.5%), oleic acid, methyl ester (15.007%), palmitic acid, methyl ester (8.208%).

Conclusion: Study concludes that in fixed oils, linoleic acid constitutes the major portion while oleic acid and palmitic acid also contributes in small quantity.

Peer Review History:

Received 4 February 2018;   Revised 11 March; Accepted 14 April; Available online 15 May 2018

Received file:        Reviewer's Comments:

Average Peer review marks at initial stage: 5.5/10

Average Peer review marks at publication stage: 7.5/10

Reviewer(s) detail:

Dr. Tanveer Ahmed Khan, Hajvery University, Lahore, Pakistan, tanveerahmedkhan754@gmail.com

Prof. Dr. Ali Gamal Ahmed Al-kaf, Sana'a university, Yemen, alialkaf21@gmail.com