SUGAR SYRUP AS A CYTOLOGICAL FIXATIVE: A COMPARATIVE STUDY WITH 95% ETHANOL DURING ROUTINE PAPANICOLAOU STAINING

Ussi Hamza Ussi; John Tongolani Mdee; Said Salim Said; Tasnim Thabit Salum; Chifundo Makweza; George Chikondi Samu; Suleiman Masoud Suleiman; Amos Rodger Mwakigonja

doi:10.22270/ujpr.v11i2.1538

Ussi Hamza Ussi School of International Education, Southern Medical University, China. School of Health & Medical Science, The State University of Zanzibar, Suza, Tanzania.
John Tongolani Mdee Department of Pathology, Kilimanjaro Christian Medical Centre (KCMC), Tanzania.
Said Salim Said Zanzibar Food and Drug Agency, Tanzania.
Tasnim Thabit Salum School of Health & Medical Science, The State University of Zanzibar, Suza, Tanzania.
Chifundo Makweza Queen Elizabeth Central Hospital, Malawi, Tanzania.
George Chikondi Samu School of Public Health, Southern medical University, China
Suleiman Masoud Suleiman Chief Government Chemist Laboratory Agency, Ministry of Health, Zanzibar, Tanzania.
Amos Rodger Mwakigonja Pathology Department Muhimbili University of Health and Allied Sciences, Tanzania.

10.22270/ujpr.v11i2.1538

Keywords:

Alternative fixative, cytology, papanicolous stain, pap smear, cytologicalfixative, 30% sugar syrup fixative, 95% ethanol fixative

Abstract

Background and Objective: Fixation is an initial and important step in cytology for microscopic examination in cytopathological techniques, which ensures the preservation of cell morphology and structures. The routine cytological fixative at Muhimbili National Hospital (MNH), Central Pathology Laboratory (CPL), is 95% ethanol, which causes shrinkage of the cell which disrupts the physical structure of almost any type of membrane, including the plasma membrane, membrane of cell organelles and liposomes. Objective of present study was to assess the effectiveness of 30% sugar syrup as a cytological fixative as compared to 95% Ethanol in Papanicolaou staining procedures in Cytology unit at MNH.

Methods: The study design was cross-sectional prospective study conducted at MNH in histopathology laboratory from April to August 2023. Whereby Thirty cytological samples were obtained from different bodily fluids, including pleural (n=12), ascetic (n=10), peritoneal (n=5), and pericardial (n=3). Whereby nuclear staining, cytoplasmic staining and cell morphology were examined under the light microscope at X40 magnification.

Results: A paired study of thirty specimens revealed that two fixatives created bad quality in two specimens (6.7%), ethanol alone produced good quality in eight specimens (26.7%), sugar syrup alone produced good quality in two specimens (6.7%), and both fixatives produced good overall staining quality in eighteen specimens (60%). While 30% sugar syrup produced acceptable staining quality in 20 of 30 specimens (66.7%), 95% ethanol demonstrated superior staining quality in 26 of 30 specimens (86.7%).

Conclusion: The paired statistical analysis did not reveal a significant disparity between the two fixatives, notwithstanding the greater absolute percentage of satisfactory staining quality observed with 95% ethanol. Consequently, 30% sugar syrup may serve as a viable alternative fixative when 95% ethanol is either unavailable or unsuitable.