PREVALENCE, BIOTYPING, AND ANTIBIOTIC SUSCEPTIBILITY PROFILES OF AEROMONAS SPECIES ISOLATED FROM DIARRHOEAL PATIENTS IN SANA'A CITY, YEMEN

Sami Sultan Ahmed Abdu; Sami Sultan Ahmed Abdu; Sami Mohammed Abdo Hassan; Hassan Abdulwahab Al-Shamahy; Ibrahim Mohammed Amed Nahshal; Ibrahim Ali Al-Arashi; Amirh Abdullah Sa'aed Aljabri; Ahmed Mohammed Al-Haddad; Naif Mohammed Al-Haidary

doi:10.22270/ujpr.v11i5.1662

Sami Sultan Ahmed Abdu Medical Microbiology and Clinical Immunology Department, Faculty of Medicine and Health Sciences, Sana’a University, Yemen.
Sami Sultan Ahmed Abdu Medical Microbiology and Clinical Immunology Department, Faculty of Medicine and Health Sciences, Sana’a University, Yemen.
Sami Mohammed Abdo Hassan Medical Microbiology and Clinical Immunology Department, Faculty of Medical Sciences, Al-Nasser University, Yemen.
Hassan Abdulwahab Al-Shamahy Medical Microbiology and Clinical Immunology Department, Faculty of Medicine and Health Sciences, Sana’a University.
Ibrahim Mohammed Amed Nahshal Medical Microbiology and Clinical Immunology Department, Faculty of Medicine and Health Sciences, Sana’a University.
Ibrahim Ali Al-Arashi Medical Microbiology and Clinical Immunology Department, Faculty of Health Sciences, Thamar University, Yemen.
Amirh Abdullah Sa'aed Aljabri Medical Microbiology and Clinical Immunology Department, Faculty of Medical Sciences, Al-Nasser University, Yemen.
Ahmed Mohammed Al-Haddad Department of Medical Laboratories, College of Medicine and Health Sciences, Hadhramout University, Al-Mukalla, Yemen.
Naif Mohammed Al-Haidary Medical Microbiology and Clinical Immunology Department, Faculty of Medicine and Health Sciences, Sana’a University.

10.22270/ujpr.v11i5.1662

Keywords:

Aeromonas, antibiotic susceptibility, diarrhea, gastritis, Yemen

Abstract

Background and Aims: The genus Aeromonas is one of new and increasingly important bacterial genera which have been included as a cause of human gastritis. The aims of the study include determination of the prevalence of Aeromonas species among diarrhoeal patients, as well as biotyping, and studying the antibiotic susceptibility of the isolates.

Methods: Initially patients suffering from diarrhoea were selected from patients who attended the main hospitals in Sana'a and sent to NCPHL for stool culture. A total of 1991 diarrheal stool specimens were collected during the period of two-year period (2023–2024). In order to identify Aeromonas the stool specimens were inoculated into alkaline peptone water, Aeromonas agar and TCBS agar, and then the isolates were identified by the relevant biochemical tests.

Results: Presents data on 1,991 diarrhea patients, predominantly children aged 2 months to 9 years (34.0%). There was a slight male predominance (52.9%). The mean age was 22.4 years, with a range of 2 months to 75 years. The leading bacterial causes were non-Salmonella typhoid serogroups (25.0%), Shigella serogroups (13.0%), and Vibrio cholerae (7.7%). Moderate to low prevalence included enteropathogenic E. coli (5.1%) and Aeromonas (1.9%). Resistance rates for Aeromonas were 100% for PG-penicillin, Ampicillin-sulbactam, Oxacillin, and Ampicillin, with high resistance to nalidixic acid (84.2%).

Conclusion: Diarrheal illness in this study population heavily burdens young children and is primarily driven by non-Salmonella typhoid and Shigella serogroups.