FORMULATION AND EVALUATION OF ELASTIC LIPOSOMES OF DECITABINE PREPARED BY ROTARY EVAPORATION METHOD

Nwobodo Ndubuisi Nwobodo; Adamude Fatima Amin; Dingwoke Emeka John; Abraham Ubhenin

doi:10.22270/ujpr.v4i3.267

Nwobodo Ndubuisi Nwobodo Department of Pharmacology and Therapeutics, Enugu State University of Science and Technology, Enugu, Nigeria. Department of Pharmacology and Therapeutics, College of Health Sciences, Nile University of Nigeria, FCT, Abuja, Nigeria
Adamude Fatima Amin Department of Biochemistry, Federal University, Lafia, Nigeria
Dingwoke Emeka John Department of Biochemistry, Ahmadu Bello University, Zaria, Nigeria
Abraham Ubhenin Department of Biochemistry, Federal University, Lafia, Nigeria

10.22270/ujpr.v4i3.267

Keywords:

Decitabine, Elastic liposomes, in vitro drug release, rotary evaporation method, Span 60, Span 80

Abstract

Objective: Decitabine is a cytidine deoxynucleoside analog, which acts by inhibiting DNA methyltransferase, and is used for the treatment of acute myeloid leukemia. Decitabine has a short half-life (25 minutes), and is sensitive to harsh conditions. Elastic liposomes are an effective tool that can be used to overcome this disadvantage. Elastic liposomes also known as transfersomes are modified lipid carriers that enable drug to reach deeper skin layers and/or the systemic circulation. These vesicular formulations are several orders of magnitudes, more deformable than the standard liposomes and thus well suited for skin penetration. The objective of present study is to develop and evaluate the elastic liposomes of Decitabine so as to provide the sustained release and improve its bioavailability.

Methods: Elastic liposomes were prepared by rotary evaporation method using Span 80 and Span 60 as a surfactants. The prepared Elastic liposomes were evaluated for entrapment efficiency, vesicle size, in vitro drug release. The drug release profiles from different elastic liposomes-in-vehicle formulations were in agreement with the physicochemical properties of the formulations.

Results: Based on different parameters formulations of batch ELS1 was found to be the best formulations. Stability study was performed on the selected formulation ELS1.

Conclusion: Study concludes that Decitabine can also be formulated in the liposomal carrier which finds its best way for the topical administration.