ANTI-DIARRHEAL ACTIVITY OF ETHANOL AND CHLOROFORM SEED EXTRACT OF COLA NITIDA IN EXPERIMENTALLY INDUCED DIARRHEA

Peace Doe; Vivien Chinenye Mshelia; Duke Bright Otchere; Omar Fofana; Audrey Antwiwaa Amissah; Persis Nadine Yeboah; Harriet Naa Lamptey Mills; Ebenezer Ansah-Abrokwah; Noah Kofi Ametepey

doi:10.22270/ujpr.v4i5.312

Peace Doe Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
Vivien Chinenye Mshelia Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
Duke Bright Otchere Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
Omar Fofana Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
Audrey Antwiwaa Amissah Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
Persis Nadine Yeboah Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
Harriet Naa Lamptey Mills Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
Ebenezer Ansah-Abrokwah Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.
Noah Kofi Ametepey Department of Pharmaceutical Sciences, School of Pharmacy, Central University Ghana, P.O. Box 2305, Tema, Ghana.

10.22270/ujpr.v4i5.312

Keywords:

Anti-diarrheal, Cola nitida, gastro-intestinal motility, seeds

Abstract

Objective: Cola nitida has been used in traditional medicine to treat diverse ailments including diarrhea. This study is carried out to investigate the antidiarrheal activity of the ethanol and chloroform seed extract of Cola nitida in Wistar albino rats.

Methods: The ethanol and chloroform extracts of Cola nitida were evaluated with different doses (100mg/kg, 300mg/kg and 650mg/kg of animal weight) orally for antidiarrheal activity using castor oil induced-diarrhea, gastrointestinal motility test and castor oil-induced gastroenteropooling in Wistar albino rats. The observed activity was compared to standard antidiarrheal drug Lopermaide hydrochloride (2mg/kg) and to distilled water (10ml/kg) which served as the negative control.

Results: Cola nitida ethanol extract at 150, 300 and 650mg/kg showed 55.64%, 59.73%, and 71.34% inhibition in gastrointestinal motility respectively. A significant reduction in diarrheal episodes (p<0.0001) was also observed with 650mg/kg of both extracts showing 100% inhibition. A reduction in the volume of fluid in the small intestine was also seen, this was however not significant. The chloroform extract of Cola nitida on the other hand produced a significant reduction in volume and weight of small intestinal content (p<0.05) with 650mg/kg showing a 92.73% inhibition of intestinal fluid accumulation.

Conclusion: The ethanol and chloroform extract of Cola nitida showed anti-diarrheal activity in animal model by decreasing the frequency of defecation and by reducing gastrointestinal motility and intraluminal fluid accumulation in the intestine.