PHYTOCHEMICAL INVESTIGATION AND EVALUATION OF ANTIOXIDANT AND THROMBOLYTIC PROPERTIES OF LEAVE EXTRACTS OF GARDENIA CORONARIA BUCH-HAM

Abstract

Objective: The objective of this study was assessing the diverse pharmacological efficacies of methanolic extracts of the whole plant of Gardenia coronaria Buch-Ham This study illustrates the phytochemicals, antioxidants and thrombolytic activity which have the possibility to be used in the coming future to open a new line of investigation. The Gardenia coronaria, one of the necessary traditional medicinal plants in Bangladesh. In the rural area this plant is used by a lot of people as a treatment from various disease.

Methods: Total sum of the flavonoids was spectrophotometrically ascertained with the help of aluminum chloride colorimetric assay as total phenolic and tannin content by Folin Chiocalteu’s reagent. Antioxidant activity, ascertained by DPPH (α, α-diphenyl-β-picrylhydrazyl) free radical scavenging assay giving some comparison between the resultant activity with the standard. The thombolytic activity was assessed based on the method of minor alterations.

Results: The percentage of clot lysis was 87.58% when 100 μl of streptokinase (30,000 I.U.), and the methanolic extract displayed the least clot lysis activity (26.79%), much less compared to the standard. It can be elicited that the extract of Gardenia coronaria Buch-Ham contains antioxidant and thrombolytic activity. The potential of these activities probably because of the availability of most of the phytochemicals supporting previous claims and verify its uses as an expected folk medicine. 

                                     

Peer Review History:

Received 3 December 2019;   Revised 12 January 2020; Accepted 24 February; Available online 15 March 2020

Academic Editor: Dr. Amany Mohamed Alboghdadly, Princess Nourah bint abdulrahman university, Riyadh, amalbgadley@pnu.edu.sa

Received file:                Reviewer's Comments:

Average Peer review marks at initial stage: 4.5/10

Average Peer review marks at publication stage: 7.0/10

Reviewer(s) detail:

Dr. Gehan Fawzy Abdel Raoof Kandeel, Pharmacognosy Department, National Research Centre, Dokki, 12622,  Giza, Egypt, gehankandeel9@yahoo.com 

Dr. Marwa A. A. Fayed, University of Sadat City, Egypt, maafayed@gmail.com