INTERLEUKIN-1β LEVELS IN THE HUMAN GINGIVAL SULCUS: RATES AND FACTORS AFFECTING ITS LEVELS IN HEALTHY SUBJECTS

Omar Ahmed Ismael Al-dossary; Abdulwahab Ismail Mohamed Al-Kholani; Khaled A AL-Haddad; Mohammed Mohammed Ali Al-Najhi; Hassan Abdulwahab Al-Shamahy; Ikram Ayad Anwar Al-adhami; Khalid Aldhorae

doi:10.22270/ujpr.v7i5.838

Omar Ahmed Ismael Al-dossary Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen. Medical Microbiology and Clinical Immunology Department, Faculty of Medicine and Health Sciences, Sana’a University.
Abdulwahab Ismail Mohamed Al-Kholani Department of conservative dentistry, Faculty of Dentistry, Sana’a University, Republic of Yemen.
Khaled A AL-Haddad Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen.
Mohammed Mohammed Ali Al-Najhi Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Sana'a University, Yemen. Orthodontics, Pedodontics and Prevention Department Faculty of Dentistry, Genius University for Sciences & Technology, Dhamar city, Republic of Yemen.
Hassan Abdulwahab Al-Shamahy Departement of Basic Sciences, Faculty of Dentistry, Sana’a University, Republic of Yemen. Medical Microbiology department, Faculty of Medicine, Genius University for Sciences & Technology, Dhamar city. Medical Microbiology and Clinical Immunology Department, Faculty of Medicine and Health Sciences, Sana’a University.
Ikram Ayad Anwar Al-adhami Biochemistry Department, Faculty of Medicine and Health Sciences, Sana’a University, Republic of Yemen.
Khalid Aldhorae Department of Orthodontics, college of Dentistry, University of Ibn al-Nafis for medical sciences, Sana’a.

10.22270/ujpr.v7i5.838

Keywords:

gingival crevicular fluid (GCF), Interleukin 1β (IL-1β), normal level, pro-inflammatory cytokine

Abstract

Background and objective: Gingival crevicular fluid (GCF) affords a exceptional window for investigation of periodontal condition as the levels of inflammatory mediators, which consequences owing to the increased local destruction of connective tissue structural elements. This study aimed to explore the interleukin 1β (IL-1β)) levels in the human gingival sulcus in healthy normal people; and the effect of host factors as age , gender, type of tooth used in pro-inflammatory biomarkers.

Methods: Eighty seven patients, 54 (62.1%) female and 33 (37.9%) male (aged 12–34 years; mean 19.58±4.4 years), participated in this study. Each subject underwent a session on professional oral hygiene and received oral hygiene instructions. Gingival crevicular fluid (GCF) sampling was conducted (baseline). GCF was collected from the Central incisor, the Lateral incisor, the Canine, the First premolar and the second premolar in this study.

Results: In total, the mean±SD of central incisor IL-1β was 32.16±4.83 pg/ml, with a mode equal to 28.01 pg/mL, the median was 32.71 pg/mL, and ranged from 20.98 to 41.25 pg/ml with the 75% interquartile range (IQR) equal to 35.94 pg/ml. For males the mean±SD of central incisor IL-1β was 31.6±5.51 pg/ml VS 32.5±4.4 pg/ml of females. For the lateral incisor, canine, first premolar, second premolar:

Conclusion: This study provides the upper limit of normal values for interleukin 1β (IL-1β) levels for subjects aged 12–34 years in the GCF. These upper limits of normal values will guide dentists in Yemen when they consider the diagnosis of periodontal disease, as well as its role during orthodontic tooth movement where they play important role in osteocyte activities (e.g, osteoclasts and osteoblasts), and will provide useful baseline data for future studies of interventions against periodontal disease, and teeth movement by orthodontics appliances, in Yemen.

Peer Review History:

Received: 9 August 2022; Revised: 13 September; Accepted: 20 October; Available online: 15 November 2022

Academic Editor: Dr. Emmanuel O. Olorunsola, Department of Pharmaceutics & Pharmaceutical Technology, University of Uyo, Nigeria, olorunsolaeo@yahoo.com

Received file: Reviewer's Comments:

Average Peer review marks at initial stage: 5.0/10

Average Peer review marks at publication stage: 7.0/10

Reviewers:

Dr. Rima Benatoui,Laboratory of Applied Neuroendocrinology, Department of Biology, Faculty of Science, Badji Mokhtar University Annaba, Algeria. benattouiryma@gmail.com

Dr. Gulam Mohammed Husain,, National Research Institute of Unani Medicine for Skin Disorders, Hyderabad, India, gmhusain@gmail.com