IN-VIVO ANTIDIABETIC POTENTIALS AND TOXICOLOGICAL EFFECT OF DENNETTIA TRIPETALA SEEDS ON ALLOXAN-INDUCED DIABETIC MALE ALBINO RATS

Prince Ogochukwu Alaebo; Anthony Cemaluk Chinedum Egbuonu; Ngozi Kalu Achi; Chiemeziem Adanma Obike; Jacinta Chigozie Ukpabi-Ugo; Obiajulu Christian Ezeigwe; Uchechukwu Daniel Chukwu; Chukwuka Enyinna Wisdom

doi:10.22270/ujpr.v9i2.1082

Prince Ogochukwu Alaebo Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B. 7267. Umuahia, Abia State, Nigeria.
Anthony Cemaluk Chinedum Egbuonu Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B. 7267. Umuahia, Abia State, Nigeria.
Ngozi Kalu Achi Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B. 7267. Umuahia, Abia State, Nigeria.
Chiemeziem Adanma Obike Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B. 7267. Umuahia, Abia State, Nigeria.
Jacinta Chigozie Ukpabi-Ugo Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B. 7267. Umuahia, Abia State, Nigeria.
Obiajulu Christian Ezeigwe Department of Biochemistry, Faculty of Bio-Sciences Nnamdi Azikiwe University, P.M.B. 5025. Awka, Anambra State, Nigeria.
Uchechukwu Daniel Chukwu Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B. 7267. Umuahia, Abia State, Nigeria.
Chukwuka Enyinna Wisdom Department of Biochemistry, College of Natural Sciences, Michael Okpara University of Agriculture, Umudike, P.M.B. 7267. Umuahia, Abia State, Nigeria.

10.22270/ujpr.v9i2.1082

Keywords:

Dennettia tripetala, Diabetes, Glibenclamide, Lipid, Rats

Abstract

Background: Hyperglycemia and dyslipidemia are hallmarks of diabetes mellitus. Diabetes mellitus is a complex metabolic disorder characterized by disturbances in carbohydrates, protein, and lipid metabolism due to insufficient insulin production.

Aim: Present study was aimed to estimate the effects of Dennettia tripetala seed methanol extract (DTSE) on blood sugar and toxicological effect in alloxan-induced diabetes models using male wistar rats.

Methods: A total of thirty (30) male albino rats were divided into six distinct groups. Group A served as normal control group, where rats were neither induced nor treated. Group B acted as the negative control group, where rats were induced but not treated. Group C served as the positive control group, where rats were induced and treated with glibenclamide. Group D consisted of rats that were induced and treated with 100 mg of DTSE. Group E included rats that were induced and treated with 200 mg of DTSE. Lastly, group F comprised rats that were induced and treated with 400 mg of DTSE. Liver and kidney functions were determined using established analytical procedures. Blood samples were collected through ocular puncture for the evaluation of biochemical parameters.

Results: Blood glucose level in all the alloxan-induced diabetic rats treated with DTSE showed a relative significant (p<0.05) reduction when compared with the controls. Histological investigation of diabetic rat’s liver and kidney indicated degradation of normal tissue architecture, however after the treatment with DTSE minor reparative alteration were seen.

Conclusions: The study suggests that DTSE possesses a hypoglycemic effect, hepatoprotective and anti-atherogenic effect but has toxicological effect on the kidney of the rats treated with high dose of DTSE as pathological changes were elicited in the organ of the rats.

Peer Review History:

Received 6 February 2024; Revised 9 March 2024; Accepted 20 April; Available online 15 May 2024

Academic Editor: Dr. A.A. Mgbahurike, University of Port Harcourt, Nigeria, amaka_mgbahurike@yahoo.com

Average Peer review marks at initial stage: 6.0/10

Average Peer review marks at publication stage: 8.0/10

Reviewers:

Ahmad Najib, Universitas Muslim Indonesia, Makassar, Indonesia, ahmad.najib@umi.ac.id

Dr. Sangeetha Arullappan, Universiti Tunku Abdul Rahman, Malaysia, sangeetha@utar.edu.my

Mayada M. El-Azab, Phytochemistry Unit, Department of Medicinal and Aromatic Plants, Desert Research Center, Cairo, Egypt. drmayadaelazab2020@gmail.com