DETERMINATION OF TANNIN CONTENT AND ANTIBACTERIAL ACTIVITY OF CHROMOLAENA ODORATA L.

  • Rahmawati Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muslim Indonesia, Indonesia.
  • Muzakkir Baits Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muslim Indonesia, Indonesia.
  • Tadjuddin Naid Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muslim Indonesia, Indonesia.
  • Nurul Azmi Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muslim Indonesia, Indonesia.
  • Raudhia Wulandari Rumata Laboratory of Pharmaceutical Chemistry, Faculty of Pharmacy, Universitas Muslim Indonesia, Indonesia.
10.22270/ujpr.v9i6.1235

Keywords:

Chromolaena odorata L., Folin-ciocalteu, tannins, TLC bioautography

Abstract

Aims and objectives: To ascertain the antibacterial capability using TLC-bioautography and the tannin content using the UV-Vis spectrophotometric method in the ethanol extract of kopasanda (Chromolaena odorata L.) leaves.

Methods: Tannins quantitatively utilizing the UV-Vis spectrophotometric technique using the folin ciocalteu reagent at a wavelength of up to 687 nm. Antibacterial potential against gastrointestinal infection-causing bacteria (Salmonella typhi, Vibrio cholerae, Escherichia coli, and Shigella dysentriae) is assessed using TLC-bioautography technique.

Results: The tannin content of the ethanol extract of kopasanda leaves (Chromolaena odorata L.) is 41.9064±0.26 mgTAE/g extract. Escherichia coli, Shigella dysentriae, Salmonella typhi, and Vibrio cholerae, respectively, create inhibitory zones in the antibacterial potential test that yields 7, 8, 8, and 7 stains. Tannins, flavonoids, alkaloids, and saponins are believed to be among the chemicals found in the TLC results obtained with stain spraying reagents 1, 3, 5, and 4, respectively.

Conclusions: The average tannin concentration of the ethanol extract of kopasanda leaves (Chromolaena odorata L.) is 41.9064±0.26 mgTAE/g extract, and it has antibacterial properties against germs that cause gastrointestinal illnesses.

                 

Peer Review History:

Received 22 September 2024;   Reviewed 3 November; Accepted 15 December; Available online 15 January 2025

Academic Editor: Dr. Amany Mohamed Alboghdadlyorcid22.jpg, Ibn Sina National College for Medical Studies in Jeddah, Saudi Arabia, amanyalboghdadly@gmail.com 

Average Peer review marks at initial stage: 6.0/10

Average Peer review marks at publication stage: 7.0/10

Downloads

Download data is not yet available.
Crossmark
Statistics
11 Views | 2 Downloads
Dimension Citations

Published

2025-01-15

How to Cite

Rahmawati, M. Baits, T. Naid, N. Azmi, and R. W. Rumata. “DETERMINATION OF TANNIN CONTENT AND ANTIBACTERIAL ACTIVITY OF CHROMOLAENA ODORATA L”. Universal Journal of Pharmaceutical Research, vol. 9, no. 6, Jan. 2025, doi:10.22270/ujpr.v9i6.1235.

Issue

Section

Research Articles
Share |