ANTIMICROBIAL EFFECT OF OIL EXTRACT OF MERIANDRA BENGHALENSIS LEAVES FROM YEMEN

Abduallah A. A. Ali; Ali G. Al-kaf; Mohammed AN Abbas; Ziad M. A. Alseraji; Abdulnaser S. A. Aklan; Bashar L. Ahmed

doi:10.22270/ujpr.v9i3.1113

Abduallah A. A. Ali Department of Medicinal, Analytical and Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Sana’a University. Pharmacy section, Medical Sciences Department, Amran Community College, Republic of Yemen.
Ali G. Al-kaf Department of Medicinal, Analytical and Pharmaceutical Organic Chemistry, Faculty of Pharmacy, Sana’a University.
Mohammed AN Abbas Pharmacy Department, Aljamiaah Institute for Medical Science, Republic of Yemen.
Ziad M. A. Alseraji Pharmacy section, Medical Sciences Department, Sanhan Community College, Republic of Yemen.
Abdulnaser S. A. Aklan Pharmacy Department, Al-Hikma University, Republic of Yemen
Bashar L. Ahmed Pharmacy Department, Aljamiaah Institute for Medical Science, Republic of Yemen.

10.22270/ujpr.v9i3.1113

Keywords:

Antimicrobial activity, essential oils, antimicrobial activity, oil extract, Meriandra benghalensis

Abstract

Background: Twenty three genera and twenty three species of the Lamiaceae family are native to Yemen. The greatest antibacterial efficacy against a wide range of pathogens, including Candida albicans, Staphylococcus aureus, Aspergillus fumigatus, and Escherichia coli was demonstrated by Lamiaceae essential oils. The primary components of Lamiaceae essential oils, including as carvacrol, thymol, p-cymene, 1,8-cineole, and caryophyllene, may be responsible for these characteristics.

Method: The plant green branches and new leaves were sliced into little bits. A Clevenger-style all-glass equipment was used to hydro distills the essential oil for five hours in order to separate it from each component. After being moved to a glass vial with a screw top, each oil was dried (with Na₂SO₄) and kept at 4°C in the dark until analysis. For assessing antibiotic sensitivity, a variety of bacterial and fungal strains kept in stock culture at the Al-Mamoun laboratories center were utilized, including: Gram-positive bacteria (Staphylococcus aureus), Gram-negative bacteria (Klebsiella pneumonia, Escherichia coli) and Fungi Candida albicans. In dichloromethane (DCM), the essential oil that had been previously synthesized was diluted 1/5, 1/3, and 1/ 1 v/v.

Result: The plant has significant effect on Staphylococcus aureus, Escherichia coli, Klebsiella pneumonia and, Candida albicans. In addition, the extract has high effect on Candida albicans than on bacteria. And their more effect on Klebsiella pneumoniae than Escherichia coli.

Conclusions: Result showed the plant has significant effect on staph. Aureus, E coli, Klebsielle, Candida albicans. The extract has high effect on Candida albicans than bacteria. Also the extract has high effect on Klebsiella than E. coli. The oil extract has more effect on gram negative than gram positive.