ANTILEISHMANIAL EFFICACY OF HERACLEUM PERSICUM DESF. EX. FISCH. EXTRACTS AGAINST LEISHMANIA TROPICA PROMASTIGOTES

Gamze Ozgun; Husniye Kayalar; Fatih Alaylı; İbrahim Cavus; Fatih Dönmez; Ahmet Ozbilgin

doi:10.22270/ujpr.v9i4.1154

Gamze Ozgun Manisa Provincial Health Directorate Merkezefendi State Hospital, Manisa, Turkey.
Husniye Kayalar Ege University Faculty of Pharmacy Department of Pharmacognosy, Bornova, Izmir, Turkey.
Fatih Alaylı Ege University, Graduate School of Health Sciences, Izmir, Turkey.
İbrahim Cavus Manisa Celal Bayar University, Faculty of Medicine, Department of Medical Parasitology, Manisa, Turkey.
Fatih Dönmez Van Yuzuncuyil University, Faculty of Pharmacy, Department of Biochemistry, Van, Turkey.
Ahmet Ozbilgin Manisa Celal Bayar University, Faculty of Medicine, Department of Medical Parasitology, Manisa, Turkey.

10.22270/ujpr.v9i4.1154

Keywords:

Antileishmanial, Heracleum persicum, Leishmania tropica, promastigotes

Abstract

Background: Heracleum persicum known as “suhotu” is widely distributed in Adana, Hakkari Van and used in the production of famous Van herb cheese. The antioxidant, antiinflammatory, antidiabetic, antiepileptic, hepatoprotective, and antifungal properties were previously investigated on H. persicum extracts.

Objectives: The purpose of the present study was to comparatively evaluate the in vitro antileishmanial potential of H. persicum extracts prepared by solvents having different polarities.

Methods: The extracts of aerial parts of H. persicum were obtained by maceration, and in vitro antileishmanial efficacy was assayed on L. tropica promastigotes using CellTiter-Glo^® Cell Viability assay.

Results: The diethylether extract of H. persicum was found to be the most active extract on L. tropica promastigotes with IC₅₀ value at 151.6 µg/ml. The n-hexane extract with 160.2 µg/ml IC₅₀ value exhibited higher antileishmanial activity than methanol, ethylacetate and etanol extracts which had IC₅₀ values at 229, 343.5, and 4708 µg/ml, respectively

Conclusions: The n-hexane and the diethylether extracts of H. persicum which showed remarkable in vitro antileishmanial activities could constitute a valuable source for leishmanicidal compounds.