ANTIMICROBIAL AND ANTIOXIDANT ACTIVITY OF PSIDIUM GUAJAVA. (GUAVA) MEDICINAL PLANT LEAVES USED IN FOLK  MEDICINE FOR TREATMENT OF  WOUNDS  AND BURNS  IN HUFASH DISTRICT AL MAHWEET GOVERNORATE–YEMEN

Samir Ahmed Ali ALhaidari; Qais Abdullah; Aziza M. Taj Al-Deen; Fatima A. Al-Hadi; Anas AL Mahbashi; Ali Gamal Al-Kaf; Fouad A. Al-Dubai

doi:10.22270/ujpr.v4i2.250

Samir Ahmed Ali ALhaidari Biology Department -Faculty of Science, Sana'a University, Yemen.
Qais Abdullah Biology Department -Faculty of Science, Sana'a University, Yemen.
Aziza M. Taj Al-Deen Biology Department -Faculty of Science, Sana'a University, Yemen
Fatima A. Al-Hadi Biology Department -Faculty of Science, Sana'a University, Yemen
Anas AL Mahbashi Biology Department - Faculty of Science, Sana'a University, Yemen
Ali Gamal Al-Kaf Medicinal chemistry Department, Faculty of pharmacy, Sana'a University,Yemen.
Fouad A. Al-Dubai Biology Department, Faculty of science, Dhamar University, Yemen.

10.22270/ujpr.v4i2.250

Keywords:

Antimicrobial, antioxidative, phytochemical, Psidium guajava

Abstract

Objective: Psidium guajava (PG) belongs to the family Myrtaceae that is believed to have active components that help to treat conditions like malaria, gastroenteritis, vomiting, diarrhea, dysentery, wounds, ulcers, toothache, coughs, sore throat, inflamed gums, and a number of other condition. The objective of current study was phytochemical screening of chemical constituents of Psidium guajava extract.
Methods: In this study methanolic and aqueous extracts of one plant namely Psidium guajava, were screened for the presence of phytochemical constituents and tested for their antimicrobial and antioxidant activity.
Results: TLC tests conducted revealed Rf values in the leaves for alkaloids, Flavonoids, Tannins, Phenols and Saponins(0.96-0.97-0.99-0.97-0.99) respectively. The antimicrobial activity extracts against four bacterial isolates Staphylococcus aureus, Escherichia coli, Pseudomonas aeruginosa and Klebsiella sp. and a single fungal isolate Candida albicans with concentrations (0.5 mg/ml, and 1,0 mg/ml) of the extract were added to the disc and respective solvent was used as negative control. The antioxidative activity of leaf was evaluated by using 1,1- diphenyl-2 picrylhydrazyl (DPPH), the results showed are 88.4%, highest from standard, ascorbic acid 87.5%.
Conclusion: The qualitative phytochemical analysis revealed the results showed presence of alkaloids, terpenoids, glycosides, resins, saponins, tannins, flavonoids, phenols, and amino acid were present in the methanol extract, with absence of glycosides, and amino acids in the aqueous extracts in leaves plant.