EVALUATION OF ANTIOXIDANT ACTIVITY OF EXTRACT COMBINATION OF GINGER AND LEMONGRASS
Keywords:
Combination, Cymbopogon citratus, DPPH assay, free radical, Zingiber officinaleAbstract
Aim and objective: This study to determine the antioxidant activity of the combination of ginger (Zingiber officinale) and lemongrass (Cymbopogon citratus) on reducing DPPH (2,2-diphenyl-1-picrylhydrazyl).
Methods: Quantitative measurement of the antioxidant activity of ginger and lemongrass extracts, as well as a combination of both with a concentration ratio of 1:1, 2:1, 1:2 using a UV-Vis spectrophotometer at a wavelength of 516 nm to determine the IC50 value.
Results: IC50 value of ginger extract 10.76 µg/mL, lemongrass extract 26.69 µg/mL, IC50 value 6.69 µg/mL for combination with concentration ratio 1:1, IC50 value 3.32 µg/mL for combination with concentration ratio 2:1. IC50 value 9.34 µg/mL for combination with concentration ratio 1:2
Conclusions: The combination of ginger and lemongrass extracts has synergistic antioxidant activity that can increase the antioxidant effect from the strong category to very strong category.
Peer Review History:
Received 17 October 2024; Reviewed 9 November; Accepted 18 December; Available online 15 January 2025
Academic Editor: Dr. Ahmad Najib, Universitas Muslim Indonesia, Indonesia, ahmad.najib@umi.ac.id
Average Peer review marks at initial stage: 5.5/10
Average Peer review marks at publication stage: 7.0/10
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